Leica TCS SP8;

Nikon A1/C2;

Olympus FV1000/FV1200/FV3000/FVMPE-RS;

Zeiss LSM 710/LSM 780;

这些共焦显微镜都可以轻松升级到荧光寿命成像FLIM / FFS功能。

采用光子计数检测，数据采集可选TCSPC的时域技术。

升级后，您可以获得：

1. 1ps的时间分辨率

2. 100ps-100ms的数据采集能力；

3. 8通道的检测能力；

4. 65MHz/channel

5. NDD双光子检测器；

FFS功能包括：

Fluorescence Correlation Spectroscopy (auto- and cross-correlation FCS)

Photon Counting Histogram (PCH)

FFS measurements at target XYZ locations in an image

FLCS, Fluorescence Lifetime Correlation Spectroscopy

Number & Brightness (N&B)

Figure 1. Schematics of the upgrade package for the Leica TCS SP8 Frequency-Domain Confocal Microscope.  The part to the right includes the instrument components (PC, control electronics, scanner and laser launcher). The left part of the schematics includes the components provided by ISS with the upgrade package.